From routine analysis to research,
employing the highest level of sensitivity in the world!
Cat. No. SZ-220-92331-20
The essence of fluorescence analysis is sensitivity. With a high-throughput optical system that employs a blazed holographic grating, photomultiplier and digital signal processing, the RF-5301PC provides the highest level S/N ratio attainable, making it ideal for applications in such areas as pharmaceuticals and pharmacology, food science, biochemistry, and environmental monitoring.
Features
High-speed Scanning
Measure a spectrum in seconds with high-speed scanning up to 5,500 nm/min. And since monochromator slewing is about 20,000nm/min, setting of two or more wavelengths can be performed quickly and easily.
High Resolution and Extended Range
The bandpass can be set as narrow as 1.5nm, enabling users to distinguish fluorescent peaks from the background emission. The wavelength range of 220 to 750nm can be extended to 900nm.
Vertical Optics with Dynode Feedback
Assures a high S/N ratio, and enables analysis using very small sample volumes. The dynode feedback enhances performance by raising or lowering negative high voltage to the detector in response to different wavelengths.
Built-In Performance Checks
Operating conditions are automatically verified, and other features help maintain the instrument in its optimum condition.
Automatic Shutter
When measurement ceases, the automatic shutter closes immediately, protecting the sample from photo-decomposition.
Software
Meeting all your fluorescence research needs, from teaching to method development and quality assurance, Shimadzu’s Windows-based software makes operation intuitive for beginners and experienced users alike. The software offers powerful data processing functions, one-touch operation, onscreen data manipulation features, easy & convenient report generation, and the following measurement modes:
- Quantitative Measurement Mode
- Spectrum Measurement Mode
- Time Course Measurement Mode
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Hardware Specifications |
| Light Source |
150W Xenon lamp. Ozone resolving type lamp housing. |
| Excitation and emission monochromators |
Concave, blazed holographic grating, F/2.5, 1300 grooves/mm. |
| Wavelength scale |
220-900nm. |
| Measuring wavelength range |
220-750nm and 0 order as standard. 220-900nm with the optional R928 photomultiplier. |
| Spectral bandwith |
6-step selection of 1.5, 3, 5, 10, 15 and 20nm. (6nm bandwith is available for half sample height on the excitation side only.) |
| Wavelength accuracy |
±1.5nm. |
| Light source compensation |
Dynode feedback system with monochromatic light monitoring. |
| Sensitivity |
The S/N ratio is 150 or higher for the Raman line of distilled water (350nm excitation wavelength, 5nm spectral bandwidth, and 2 second response for 98% of the full scale). |
| Wavelength scanning |
7-step selection of Survey (about 5500nm/min), Super (about 3000nm/min), Very Fast, Fast, Medium, Slow and Very Slow. |
| Wavelength slewing speed |
About 20,000nm/min. |
| Response |
8-step selection of 0.02, 0.03, 0.1, 0.25, 0.5, 2, 4, and 8 seconds for 98% of the full scale. |
| Sensitivity selection |
2-steps of HIGH and LOW. (The sensitivity at HIGH is about 50 times that of LOW.) |
| Interface |
RS-232C interface, interface for autosampler, and interface for sipper unit. |
| Dimensions and weight |
667W x 530D x 270H mm; 43 kg. |
| Power requirements |
100, 120, 220, 240V; 50/60Hz; 400VA. |
| Operational temperature range |
15-35°C |
| Operational humidity range |
40-80% (Below 70% with temperature higher than 30°C). |
| Software Specifications |
| Measurement |
Excitation, emission and synchronous spectrum measurement, time-course measurement, quantitation, automatic search of optimal excitation and emission wavelengths |
| Data processing |
Arithmetic calculation between spectra and between a spectrum and a constant, smoothing, 1st through 4th derivatives, 1/Y, logarithmic conversion, data printout (with or without activity value computation), peak pick, point pick, area calculation, averaging (in quantitation), generation of calibration curves of 1st through 3rd order. |
| Filing |
Save, recall, and delete data. Conversion into ASCII or CSV formats. |
| Data output |
Automatic scale adjustment, readout of data at user-specified point, data printout (preview function provided), and selection of colors and types of curves. |
| Maintenance |
Macro ability for signal-to-noise ratio, monitoring of the run time of light source lamp. |
| User interface |
ToolBox (assign icons for commonly used menu commands), Online Display, and Macro ability. |
| Other functions |
Data exchange via clipboard, auto response control, automatic shutter. |
| PC requirements |
IBM-PC/AT or 100% compatible; 486 or higher CPU; 16 Mbyte or larger main memory. Operates on MS-Windows version 95 or higher. |
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