Two-Compartment Bioreactors - Efficient Expression of Recombinant Proteins

NEW! CELLine adhere (AD) is specifically adapted to promote growth of anchoragedependent cells (e.g. HEK, BHK, CHO cells). The bioreactor contains a woven, polyethylene terephtalate (PET) matrix in the cell compartment providing an ideal surface for cell attachment.

CELLine classic (CL) is ideal for laboratory scale applications using suspension cells or adherent cells in connection with microcarriers. The unit is optimised for cultivation of hybridomas and many other cell types (e.g. CHO, NS0, SF cells).

Features:

Easy to operate

Due to its uncomplicated design working with the CELLine bioreactor is as simple as with any standard tissue culture flask. The system operates independently of any complicated control technology and works without any pump systems or agitation devices. Easily and securely stackable the CELLine occupies the minimum of space in any standard CO 2 incubator.

Cost efficient

CELLine has been designed to bring considerable cost savings to cell cultivation. The bioreactor prevents you from the need of making high capital investments into expensive cell culture instrumentation or handling large amounts of cell culture disposables in order to be able to obtain milligram amounts of a designated protein. High production yields obtained with CELLine paired within the relatively small culture volume also contribute to cost and time savings in downstream processing steps. Further, CELLine allows to make significant savings in media supplements expenses, when the addition of serum or other synthetic additives is limited to only the cell compartment.

Milligram production of protein

The high cell densities obtained within CELLine promote high expression levels of biomolecules. Product concentrations retained in the cell compartment of the bioreactor are in most cases 50 to 100 times above what is found in standard cell culture disposables. Average protein yields obtained from a single bioreactor range
between 40 to 100 milligrams every two weeks.

In vivo type cultivation

Successful cultivation of different cell types demands not only skilful handling, but also depends to a large extent on the chosen cultivation system. CELLine technology closely mimics the physiological conditions within the body enabling production of drastically increased cell densities and more organotypic cell morphologies, sometimes even to the extent of 3-dimensional cell growth.

Consistent product quality

Working with classic cell culture techniques protein contamination resulting from serum or cell protein are often a significant part of the total protein fraction. Due to considerably high protein concentrations obtained in CELLine the background of contaminating proteins is much less significant. Further, when using the CELLine to express monoclonal antibodies in hybridomas, product concentrations obtained are in the same range as observed in the ascitic fluid of mice, but free of any contamination from mouse immunoglobulins.

Visibility

The transparent design of the CELLine classic Bioreactor enables visual conrol and micro-scopic observation of the growth process to be carried out.

Animal welfare

With CELLine, INTEGRA Biosciences provides a disposable bioreactor which is competitive in cost and performance to the production of monoclonal antibodies in the ascitic fluid of mice. Over recent years, CELLine technology has been successfully adopted worldwide for the production of monoclonal antibodies and thereby has contributed diminishing the use of laboratory mice.
In addition, working with CELLine, scientists also have a tool to optimise and significantly reduce the use of fetal bovine serum compared to cell cultivation in standard, homogeneous cell culture disposables.

Specifications:

CELLine Two-Compartment Bioreactors are manufactured from optically clear virgin polysterene with a cell compartment base made of a molded silicone providing a 0.2 µm vent barrier. The compartments are separated by a 10 kDa semi-permeable, cellulose acetate membrane which are individually pressure tested for integrity. The bioreactors are stackable stabilized by specific interlocks. The units are shipped individual packed in easy to open, medical grade blister packaging, sterilized by gamma irradiation and are non pyrogenic.

	CELLine CL 350	CELLine CL 1000	CELLine AD 1000
Size: L x W x H (mm)	190 x 95 x 62	275 x 180 x 80	275 x 180 x 80
Weight (g)	185	334	336
Medium compartment cap	28 mm vented (0.2 µm), green polypropylene cap with polypropylene liner	38 mm vented (0.2 µm), white polypropylene cap with polypropylene liner	38 mm vented (0.2 µm), black polypropylene cap with polypropylene liner
Cell compartment cap	24 mm polypropylene cap with polyethylene liner	28 mm polypropylene cap with polyethylene liner	28 mm polypropylene cap with polyethylene liner
Cell compartiment inlay	none	none	PET matrix inlay
Microscopic viewing (inverted microscope)	center window requires objective working distances of 2.5 mm	center window requires objective working distances of 2.5 mm	limited visibility due to inlay matrix
Vertical and horizontal volume markings	50 - 350 ml	100 - 1000 ml	100 - 1000 ml

Product Name	Description	Quantity/Case	Item No.
CELLine CL 350	Disposable Two-Compartment Bioreactor for suspension cells, 350 ml media volume, 5 ml culture volume	5	WWM-90010
CELLine CL 1000	Disposable Two-Compartment Bioreactor for suspension cells, 1000 ml media volume, 15 ml culture volume	3	WWM-90005
CELLine AD 1000	Disposable Two-Compartment Bioreactor with matrix inlay for anchorage-dependent cells, 1000 ml media volume, 15 ml culture volume	3	WWM-90025