The unique multifloor tissue culture flasks are built with one or two floors, expanding the growth surface of a standard 150cm 2 flask to 285cm 2 or 420cm 2 respectively. These new cell culture flasks are particularly suitable for the cultivation of large quantities of adhesive cells. Cell detachment is obtained via a standard enzymatic process. Each floor includes 3 rows of 3 slots, which ensures an adequate medium flowing over all cell surfaces, and also guarantees appropriate gas exchange through the media.

The floor slots, along with the double counter-slope design under each floor of the flask, aid in exhausting air bubbles that occur during filling. In the two-floor flask, the rows of slots in each floor are designed to be non-vertically aligned with each other to allow cells suspended in the media during the seeding phase to lay in a homogeneous way to each cell culture surface. To aid in locating cells during microscopic examination, each floor includes transversal graduation lines, which divide the surface into 12 areas. Each floor carries an engraved digit (1 or 2) corresponding to that floor at the crossing point.