Our Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process
that exdudes the use of organic solvents, yielding a cleaner end-product
with a significantly reduced environmental impact.
Its low EEO (≤O.13-mr) promotes increased electrophoretic mobility,
yielding improved resolution and shorter run times. This also allows
macromolecules and larger particles (subcellular fragments, viruses, etc.)
to migrate more freely through the gel matrix.
The consistently low EEO
also provides a reduction in band distortion (caused by counterflow) that
can result from the presence of excessive sulfate-rich negative ions.
Agarose LE is widely used for nucleic acid electrophoresis (analytical
and preparative), protein electrophoresis (including radial
immunodiffusion) and various blotting protocols. It is easily soluble,
free of nucleases, and easy to use. It is highly transparent (forms a
clear, colourless solution at 1g:100ml H2O), and exhibits exceptionally
low absorption of chemical staining agents. Pore size can be
adjusted by simple modifications to the concentration ratio.
Formulated for high gel strength and integrity, Agarose LE exhibits
exceptional thermal stability and mechanical resistance, ensuring
safe, easy handling, regardless of whether a denaturing agent
has been added.
- Multi-purpose, high purity
- Enhanced resolution and clarity
- Reduced background
- Low EEO/increased electrophoretic mobility
- RNase, DNase, Protease-free
- High hysteresis, improved thermal stability
- Used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp)
Specifications
Moisture: |
≤7% |
| EEO (electroendosmosis): |
≤0.13 -mr |
| Sulfate: |
≤0.20% |
| Gelling Temp (at 1.5%): |
36° ± l.5°C |
| Melting Temp (at 1.5%): |
88° ± 1.5°C |
| Gel Strength (at 1.0%): |
1200g/cm2 |
| Gel Strength (at 1.5%): |
2500g/cm2 |
| DNase, RNase: |
None detected |
| Endonuclease: |
None detected |
| Protease: |
None detected |
|
Cat. No.
|
Description
|
|
Agarose LE, Organic Solvent Free, 500g
|
DEL-5000
|
|
Agarose LE, Organic Solvent Free, 100g
|
DEL-5002
|
| Agarose LE, Organic Solvent Free, 25g |
DEL-5001 |
|