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Mandel Agarose LE
Mandel AgaroseOur Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that exdudes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact.

Its low EEO (≤O.13-mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix.
The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colourless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.

  • Multi-purpose, high purity
  • Enhanced resolution and clarity
  • Reduced background
  • Low EEO/increased electrophoretic mobility
  • RNase, DNase, Protease-free
  • High hysteresis, improved thermal stability
  • Used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp)

Specifications

Moisture:

≤7%

EEO (electroendosmosis): ≤0.13 -mr
Sulfate: ≤0.20%
Gelling Temp (at 1.5%): 36° ± l.5°C
Melting Temp (at 1.5%): 88° ± 1.5°C
Gel Strength (at 1.0%): 1200g/cm2
Gel Strength (at 1.5%): 2500g/cm2
DNase, RNase: None detected
Endonuclease: None detected
Protease: None detected

 

Cat. No.

Description

Agarose LE, Organic Solvent Free, 500g

DEL-5000

Agarose LE, Organic Solvent Free, 100g

DEL-5002

Agarose LE, Organic Solvent Free, 25g DEL-5001
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