Cat. No. AMR-N643-KIT
A simple 2 part RNA purification procedure that combines an initial extraction step with RiboZol™ RNA Extraction Reagent, followed by spin column chromatography to generate high yields of purified, concentrated total RNA. The proprietary spin column matrix binds RNA while the unwanted protein and DNA are washed through.
Features:
- RNA extraction with RiboZol™ RNA Extraction Reagent.
- Purification/concentration with a proprietary spin column matrix.
- RNA is eluted with supplied Elution Buffer.
- Excellent recovery of large RNAs such as rRNA and mRNA.
- Superior recovery of small RNAs including miRNA and siRNA.
- Compatible with downstream applications including qRT-PCR.
- Each RiboZol™ Plus RNA Purification Kit includes sufficient RiboZol™ RNA Extraction Reagent, spin columns and collection tubes for 50 isolations.
Simple, Rapid Procedure:
RiboZol™ Plus Kit enhances recovery of small RNA species. 5x106 K562 cells were isolated with RiboZol™ RNA Extraction Reagent, and further purified with RiboZol™ Plus Purification Kit Columns (lane 2-3) or Competitor’s Purification Kit Columns (lanes 4-5). Arrow indicates small RNA species that are recovered in greater abundance with the columns used in the RiboZol™ Plus RNA Purification Kit compared to Competitor’s Kit columns.
RT-qPCR using miR-21 and 5S rRNA primers. Amplification of 5S rRNA and miR-21 RNA purified with RiboZol™ Plus. RNA from 1x106 HeLa cells was extracted and purified using RiboZol ™ Plus RNA Purification Kit. 2μl RNA was used in a 20μl RT reaction with either a 5S reverse primer or miR-21 stem-loop reverse primer. 2μl of the RT reaction was analyzed by qPCR using BioRad’s iQ™ SYBR® Green Master mixes.
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