The Detector™ AP Chemiluminescent Blotting Kit is designed for the hybridization and chemiluminescent detection of biotinylated DNA or RNA probes on membranes, providing the highest sensitivity of KPL's non-radioactive nucleic acid detection systems. Ideal for detection of Northern blots or single copy genomic Southern blots, moderately expressed transcripts in just 50 ng of total RNA and single copy genes in 1-5 ug human genomic DNA can be observed in as little as 10 minutes. Applications include Southern blotting, Northern blotting, bacterial colony hybridizations, plaque hybridizations, and dot blotting.

Features and Benefits

• Signal equivalent to a 3.5 hour 32P exposure just after 2 minutes when using an RNA/DNA probe.
• Provides higher signal-to-noise than the competition.
• Chemiluminescent substrate continues to emit light for hours, allowing multiple exposures.

Kit Contents
Two sizes available; sufficient reagents provided to process 500cm3 or 2000cm3 membrane.

1. Hybridization Buffer
2. Detector Block Solution (5X)
3. Detector Block Powder
4. AP-labeled Streptavidin
5. AP Wash Solution Concentrate (5X)
6. AP Assay Buffer (10X)
7. CDP-Star Chemiluminescent Substrate

Recommended Storage Conditions
Stable for 1 year when stored at 2-8°C.