- Liquid Concentrate, Ready-to-use Proteomics Grade Buffers for Protein Isolation
- Convenient For Everyday Use as Well as in High Throughput Expression, Purification and Characterization of Recombinant Proteins
- Consistent Performance from Lot-to-lot
- Easy to Use and Economical
AMRESCO® offers three new affinity column chromatography buffers to optimize the purification and recovery of recombinant proteins. The Phosphate Chromatography Buffer is an affinity column binding buffer used in metal chelate chromatography. Two types of elution buffers are available, designed specifically for use with His-Tag fusion proteins and monoclonal antibodies. The Imidazole Chromatography Buffer is an elution buffer for nickel affinity column chromatography used to isolate His-Tag fusion proteins. The Glycine Chromatography Buffer is an elution buffer for Protein A and Protein G affinity columns, used for isolating monoclonal antibodies.
| FUSION TAG |
IMMOBILIZED
LIGAND |
GENERAL
BINDING
CONDITIONS |
GENERAL
ELUTING
CONDITIONS |
ELUTION BUFFER |
| Polyhistidine
(6X His) |
Chelated
Ni2+ or Co2+ |
Neutral, pH without
redox agents. Tag
must be accessible
in protein structure. |
> 200 mM
Imidazole, low pH |
Imidazole Buffer
(Code: M264) |
| Monoclonal
antibody |
Protein A,
Proten G
sepharose |
High ionic strength,
neutral pH |
Low pH, decrease
in ionic strength |
Glycine Buffer
(Code: M265) |
Product Description |
Concentration |
pH |
Size |
Cat. No. |
Phosphate Chromatography Buffer,
Binding Buffer |
5 X |
7.4 |
100 ml
500 ml |
AMR-M263-100ML
AMR-M263-500ML |
Imidazole Chromatography Buffer,
Elution Buffer (His-Tag) |
5 X |
7.0 |
100 ml
500 ml |
AMR-M264-100ML
AMR-M264-500ML |
Glycine Chromatography Buffer,
Elution Buffer (GST Tag) |
5 X |
3.0 |
100 ml
500 ml |
AMR-M265-100ML
AMR-M265-500ML |
Note: see Chemicals & Reagents for complete alphabetical listing of AMRESCO® products and specifications
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