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331 / 332 HPLC Pumps

Gilson’s 331 and 322 HPLC pumps are dual-piston pumps for semipreparative and preparative chromatography. They feature a variable-volume, dynamic mixer for optimized gradient performance.

Cat. No. GA-38103312

Features:

  • Milligram-level sample mass throughput per injection: 150 mg with a 20-mm bore column
  • Easy linear transposition from analytical to preparative HPLC methods on a 4 to 20 mm ID column packed with high-efficiency stationary phases
  • Solvent selection on each pump with optional four-solvent valve (up to eight solvents)
  • Control from 331 Master Pump: flow-rate gradient, four-solvent selection and step gradient (additional valve), composition gradient (one or two additional pumps), sample-injection pump, UV/VIS detector, and electrical input/output contacts with any other associated equipment
  • Keypad control: safety files (pressure limits, emergency signal and power failure); anomaly processing procedures with related outcomes; GLP functions: solvent consumption, diagnostic helps, maintenance logs, leak test, piston seal installation, mixing test and audit trail
  • UniPoint™ System Software provides control within a complete prep workstation; includes data processing and results reporting

Specifications:

Manufacturing Standards

Meet applicable Safety and EMC certification standards; UL and CE certified

Composition and Mixing

Increment: 0.1%
Dwell Volume: 372–2,372 µL, pressure independent
Gradient Linearity Range : 1–98% B for 10 mL/min and higher

Environmental Conditions

Indoor use
Altitude: up to 2000 m
Temperature range: 5–40°C
Air pressure: 75–105 kPa
Pollution degree: 1 or 2, in accordance with IEC 66
Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C

Front Panel (331 Pump)

40-character, eight-line display, adjustable contrast and screen saver; 25 functional keys, including six soft keys

Flow Rate

0.5–50 mL/min (8,700 psi)
Selectable units: µL/min and mL/min (default: mL/min)
Adjustment increment: 1 µL/min
Accuracy: bias <±1% or 1.3 µL/min (331), bias <±2% or 13 µL/min (331/332)
Repeatability: RSD <0.5% or 1 µL/min (331), RSD < 0.7% or 7 µL/min (331/332)

Liquid-Contact Materials

316L stainless steel, titanium, FEP, PTFE, PCTFE, ETFE, PEEK, UHMWPE, ruby, sapphire, and ceramics

Power Requirements

Frequency: 50–60 Hz
Voltage: 90–120 or 220–260V; mains voltage fluctuations not to exceed ±10% of the nominal voltage

Pressure

Nominal range: from 0.5 MPa (5 bar, 70 psi) up to 60 MPa (600 bar, 8,700 psi)

Pump Options

331: single-solvent master pump (w/control panel), 0.05–50 mL/min, 60 MPa (8,700 psi)
331/332: two-solvent pumping system, 0.5–50 mL/min, 60 MPa (8,700 psi)
332: third-solvent, remote controlled pump (w/o control panel), 0.5–50 mL/min, 60 MPa (8,700 psi)

Software

Via UniPoint ™ System Software and 506C interface or GSIOC slave bus

Dimensions (w x d x h)

331: 26 x 41 x 50.7 cm (10.2 x 16.1 x 20 in.)
332: 26 x 41 x 38.7 cm (10.2 x 16.1 x 15.2 in.)

Weight

35 kg (77 lbs.)

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