Gilson’s 350 Micro Pump provides precise splitless solvent delivery for nano to micro LC that delivers accurate and reproducible gradients from 300 nL/min to 50 µL/min The 350 is capable of delivering true nano flow rates without requiring a flow splitter.
Cat. No. GF-38103931
Features:
- Modular, binary pumping system with independent micro mixer optimizes separation with nano, capillary and micro columns for 1-D, 2-D and LC/MS/MS applications
- Splitless flow delivery eliminates the inaccuracy of flow splitters, while dramatically reducing solvent consumption
- Dual-piston pump with patented magnetic coupling ensures precise, accurate flow rates from 300 nL/min to 50 µL/min
- 60-nL mixer for fast gradient response times
- Pump head seal wash permits the use of buffered solvents with increased confidence and decreased maintenance
Applications:
- Nano- and micro-scale HPLC and LC/MS/MS analysis
- Two-dimensional HPLC separations for biological and drug-related assays
- MALDI-TOF plate preparation via HPLC
Specifications:
Pump Options |
Isocratic pump (350D): electrically-actuated valves, 300 nL/min to 50 µL/min, 30 MPa (300 bars, 4,350 psi), micro degasser
Gradient pumping system (350D + 350 + gradient pumping kit): electrically-actuated valves, 300 nL/min to 50 µL/min, 30 MPa (300 bars, 4,350 psi) |
Pump Heads |
Micro E head |
Number of Solvents/Pump |
One |
Flow Rate |
Gradient: 0.3–50 µL/min (4,350 psi)
Isocratic: 0.15–50 µL/min (4,350 psi)
Selectable units: nL/min or µL/min
Adjustment increment: 0.01 µL/min
Accuracy: <±1% (5–50 µL/min)
Repeatability: <0.5% for 5–50 µL/min; <1% for 0.5–5 µL/min |
Composition and Mixing |
Adjustment increment: 0.1% B for 1–50 µL/min; 1% B below 1 µL/min
Dwell volume/Effective mixing volume: 60 nL (up to 5 µL/min); 3 µL (above 5 µL/min)
Gradient linearity range: 2% at 1% B; 97% at 100% B
Accuracy: ±1%
Repeatability: <1% RSD |
Pressure |
Operating range: 0 MPa–30 MPa (300 bar, 4,350 psi)
Accuracy: <±1% or 0.1 MPa (1 bar, 15 psi)
Repeatability: <1% or 0.1 MPa (1 bar, 15 psi) |
Pump Volume |
37.8 µL (from inlet of inlet valve to outlet of outlet valve) |
Piston Stroke Volume |
39.4 µL |
Software |
UniPoint™ System Software via 506C interface |
Communication Interface |
Nine-pin GSIOC socket, three input and four output contact closures |
Front Panel |
Green POWER, yellow ON, and yellow REMOTE LED indicators; ON/OFF soft key |
Liquid Contact Materials |
316L stainless steel, titanium, hastelloy, zirconium, FEP, PTFE, PCTFE, ETFE, PEEK, and UHMWPE |
Power Requirements |
Frequency: 50–60 Hz
Voltage: 100–120 or 220–240V; mains voltage fluctuations not to exceed ±10% of the nominal voltage |
Environmental Operating Temperature |
4–40°C |
Manufacturing Standards |
Meets applicable Safety and EMC certification standards; UL and CE certified |
Dimensions (w x d x h) |
26 x 34 x 30 cm (10 x 13 x 12 in.) |
Instrument Weight |
350: 13.5 kg (30 lbs.)
350D: 15 kg (33 lbs.) |
Shipping Weight |
350: 18.5 kg (41 lbs.)
350D: 20 kg (44 lbs.) |
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