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FC 203B Fraction Collector

The FC 203B is the perfect compact fraction collector, capable of handling a wide variety of collection vessels, including microplates, vials and tubes. The built-in keypad makes setup and operation simple and efficient. Collect fractions by time, drop or peak to suit all your LC applications.

Cat. No. GA-171011

Features:

  • In peak mode, collector monitors a detector signal and identifies peaks using either an adaptive-slope algorithm or a specified millivolt threshold
  • Up to 10 programmable time windows can be added for collecting only what you want, while discarding the column’s void volume, peaks of no interest and equilibration volumes
  • Stand-alone instrument with an easy-to-use keypad, or can be controlled through your PC via UniPoint™ System Software
  • Optional 3-way diverter valve prevents contamination of collected fractions
  • Contact closure inputs are available to remotely start/advance or stop—compatible with most HPLC systems
  • Compact size fits in most fume hoods
  • Multicolumn adapters for collecting up to 18 separate channels

Specifications:

Manufacturing Standards

Meets applicable safety and EMC certification standards; UL and CE certified

3-way Valves

FC 203B/204: 3-port, PTFE, 67 µL internal volume, 3.5 µL dead volume from common port to the normally closed port, up to 20 mL/min with standard or large bore needle
FC 204: 3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 20 mL/min with standard or large bore needle

Communication Interface

RS-232 or GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices

Data Acquisition

An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC

Detector Input

±100 mV full scale

Drop Counting

Up to 9,999 drops per fraction; maximum rate: 20 drops /sec

Environmental Conditions

Indoor use
Altitude: up to 2000 m
Temperature range: 5–40°C
Air pressure: 75–105 kPa
Pollution degree: 1 or 2, in accordance with IEC 66
Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C

Event Marker

100 ms pulse

Front Panel

Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0–9 numeric hard keys; four soft keys

Maximum Collection Volume/Tube

FC 203B: 20 mL (28 x 60 mm scintillation vials with optional Code 24 rack)
FC 204: 32 mL (18 x 180 mm tubes or 25 mL (18 x 150 mm)

Maximum Fractions

FC 203B: 128 (12 x 75 mm tubes with optional Code 14 rack)
FC 204: Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)

Multiple-Column Collection

Simultaneous collection from up to 8 (FC 203B) or up to 18 (FC 204) columns with installation of optional multiple column adaptor(s)

Multiple-Cycle Operation

Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes
3-port, PTFE, 114 mL internal volume, 6 µL dead volume from the common port to the normally closed port, up to 200 mL/min with large bore needle

Number of Racks

FC 203B: Holds one rack
FC 204: Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)

Operating Modes

Drop, Manual, Peak + Drop, Peak + Time, Time

Peak Detection

Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value
Peak parameters: peak height or level, in mV and peak width at half-height, in minutes
Minimum peak height for fractionation: 0.1 mV full scale
Detector-collector delay: 0.01 minute increments

Power Requirements

Frequency: 50–60 Hz
Voltage: 100–120 or 220–240V; mains voltage fluctuations not to exceed ±10% of the nominal voltage
Current rating: 0.5A for 100–120V, or 0.25A for 220–240V

Programmable Time Units

From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution

Software

Via Gilson UniPoint™ System Software

Time-Based Programming

Up to 10 collection windows and 10 drain steps in any mode

Tube Change Time

100–250 ms (FC 203B) or 100–720 ms (FC 204), center to center, depending on rack type

Zero Power Memory

Maintains memory for a minimum of five years from date of shipment

Dimensions (w x d x h)

FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in.)
FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)

Weight

FC 203B: 5 kg (11 lbs.)
FC 204: 10.9 kg (24 lbs.)

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