A non-mutagenic, environmentally friendly alternative to Ethidium Bromide

EZ-VISION™ is a fluorescent DNA reagent that enables instant band visualization during or immediately after running your agarose gel. Supplied in AMRESCO’s 6X Loading Buffer, it forms a tight complex with the sample DNA and co-migrates with it during electrophoresis. After the run, simply place the gel on a standard UV transilluminator to instantaneously see the bands. No post-run staining or destaining is required. EZ-VISION™ provides a faster, safer, environmentally friendly alternative to Ethidium Bromide.

EZ-VISION™ staining of 1kb DNA ladder on 1% agarose gel run in TAE buffer. Markers were mixed with EZ-VISION™ and aliquots were loaded as follows: Lane 1: 1.00µg. Lane 2: 0.75µg. Lane 3: 0.50µg. Lane 4: 0.25µg. The gel was run at 135 volts for 1 hour. DNA bands were visualized by exposure to UV light.

Easy and Convenient

• Fluorescent DNA dye supplied in a 6X loading buffer.
• Simply add EZ-Vision™ to your samples and load your agarose gel.

Instant Results

• Visualize DNA instantly with a standard U.V. transilluminator.
• Very low background.
• Requires no post electrophoresis staining or destaining.
• Works with most existing filters for gel documentation.
• Very broad emission spectra with peak near 450 nm.

Better for you and the environment than ethidium bromide 

• Non-mutagenic and non-toxic.
• Non-toxic for waste disposal.
• No hazardous shipping, storage or disposal fees.

Simple, Safe and Sensitive

• Does not alter migration of DNA during electrophoresis.
• Retains fluorescent signal longer than leading competitor.
• Sensitivity similar to Ethidium Bromide at 6ng of DNA above 500 bp, and at 12ng of DNA at 50 bp.

Applications:

• All analytical applications where rapid DNA band visualization is desired*:
  • Cloning, including ligation and transformation
  • PCR
  • Southern blotting
• Teaching labs
• High throughput screening

NOW available with 3 different tracking dye options!
Each version contains the same non-hazardous fluorescent DNA dye and loading buffer.

EZ-Vision™ One, Two and Three. Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision ™ One (lane 1), EZ-Vision™ Two (lane 2) and EZ-Vision™ Three (lane3) captured with Syngene GBox-HR Gel Doc Systemusing SP filter selection. Right image: Digital camera photograph of the same gel as left image, showing the colors and migration position of the one fast migrating tracking dye of EZ-Vision™ One (lane 1), the two tracking dyes of EZ-Vision™ Two (lane2), and the three tracking dyes of EZ-Vision™ Three (lane 3).

1 tracking dye migrating at approximately 10bp in a 1% agarose gel

Cat. No
AMR-N472-Q-0.5ML
AMR-N472-KIT

2 tracking dyes migrating at approximately 4000bp and 400bp in a 1% agarose gel

Cat No.
AMR-N650-Q-SAMPLE
AMR-N650-KIT

3 tracking dyes migrating at approximately 4000bp, 400bp and 10bp in a 1% agarose gel

Cat No.
AMR-N313-Q-SAMPLE
AMR-N313-KIT 

* Please note that EZ-VISION™ may reduce the efficiency of some down-stream applications, including sequencing

EZ-Vision™ Safety Testing

EZ-Vision™ mutagenicity was determined by Ames testing of S. typhimurium with and without metabolic activation with an S-9 activation system. Sample concentrations tested did not produce a two-fold increase in the number revertants and did not meet the criteria for a potential mutagen.

EZ-Vision™ environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision™ Two and EZ-Vision™ Three were determined non-hazardous with LC50 > 750 mg/l.

Relative migration of prestained samples of dsDNA in1% agarose gel with EZVision ™, Competitor 2 Green Dye, and Ethidium Bromide. The data shows that EZ-Vision™ has much less effect on DNA migration than Ethidium Bromide or Competitor 2 Green Dye. Samples stained with EZ-Vision™ are not impeded and migrate similar to dsDNA that is stained post electrophoresis.

EZ-Vision fluorescent signal degradation in agarose gels with U.V. exposure over time. Sample DNA was stained with EZ-Vision™ or Competitor 1 Green Dye and electrophoresed on 1% agarose gels. Following electrophoresis, gels were exposed continuously to u.v. light, and the fluorescent signal quantified at 10 minute intervals using SyneGne GBox-HR Gel Doc System, presented as % of original signal. The data demonstrates that DNA stained with EZ-Vision™ retains the fluorescence approximately twice as long as Competitor I.