A non-mutagenic, fluorescent stain for denaturing agarose electrophoresis of RNA.
Non-mutagenic fluorescent dye for staining RNA bands resolved on denaturing agarose gels containing formaldehyde. The dye comigrates with the RNA samples during electrophoresis and provides immediate band visualization upon illumination with UV light.
Features:
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Supplied in a 1.5X loading buffer, RNA EZ-Vision™ dye stains RNA during the denaturation immediately prior to gel loading.
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No post run staining or destaining is needed.
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The loading buffer includes formamide and bromophenol blue tracking dye.
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A non-mutagenic alternative to ethidium bromide for RNA.
- Ideal for environments needing to reduce use of ethidium bromide.
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Sensitive to 150 ng of RNA.
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Compatible with downstream applications including western blots.
RNA Staining with RNA EZ-Vision on a 2% Agarose Gel
Total RNA was purified from K562 cells with Ribozol™ RNA Extraction Reagent. Samples were denatured at 65°C for 10 minutes in RNA EZ-Vision™ loading buffer containing either 0.003% ethidium bromide or RNA EZ-Vision™ dye. The denatured RNA was loaded onto a 2% formaldehyde denaturing agarose gel (Agarose I™, AMR- 0710) and resolved for 1.5 hours at 5.1 V/cm. Bands were visualized by UV illumination and image capture was performed with a Syngene GBox-HR Gel Doc System with a SYBR® Green filter.
Product |
Cat. No. |
| RNA EZ-VISION™ DNA Dye as Loading Buffer - Sample |
AMR-N717-Q-SAMPLE
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RNA EZ-VISION™ DNA Dye as Loading Buffer - 1.5ML |
AMR-N717-2X1.5ML |
|
