Maximize transformation efficiency by eliminating ultraviolet induced cleavage of DNA fragments.

Visual Violet™ DNA Stain is a chromogenic in-gel stain for detection of DNA fragments in agarose gels. Pre-cast into the gel, Visual Violet™ enables real time monitoring of electrophoresis and eliminates post-run staining and destaining. DNA bands can be excised from the gel by placing it on a visible spectrum (white) light box. The kit includes Visual Violet™ DNA gel stain, 200X and Visual Violet™ Loading Buffer, 6X.

Features:

• In-gel chromogenic stain for DNA in agarose gels.
• Increase transformation efficiency 3-5 fold.
• Sensitive down to 50 ng of DNA per band.
• Easy-to-use: simply add to melted agarose and cast gel.
• Compatible with downstream cloning applications.

Enhanced Transformation Efficiency with Visual Violet™

2 μg each of pUC19 and insert DNA was digested with KpnI and HindIII. Samples were divided into 1 μg aliquots, mixed with loading buffer and resolved on 0.8% agarose gels containing either ethidium bromide (EtBr) or Visual Violet™. DNA from the EtBr gel was exposed to 307 nm ultra-violet light at 70% power for 35 seconds during excision of the DNA band. DNA from the Visual Violet™ gel was excised on a white light box. After ligation of plasmids and inserts, the DNA was transformed into DH5α competent cells. 1/3 of the transformed cells were plated in LB/AMP/IPTG/X-GAL plates (A) and white colonies were counted using GeneTools software (Syngene). Data represents three separate ligation/transformations with equal amounts of either EtBr or Visual Violet™ purified DNA. Error bars represent one standard deviation.

Visual Violet™ Sensitivity

DNA (25 ng - 400 ng) was mixed with 6X Visual Violet™ Loading Buffer and applied to a 1% Visual Violet™ agarose gel. Samples were resolved for 1 hour at 5.2 V/cm in 1X TAE buffer. Image capture was performed on a white light box with digital photography.