The GenSpin Purification Kit is designed to purify high quality, PCR-ready, single stranded DNA in solution from whole blood and cultured cells in as little as 25 minutes.

Cat. No. W-WB120005

Features:

• Simple, single tube protocol. Purify single stranded DNA from whole blood and cultured cells in less than 25 minutes
• FTA technology lyses cells, denatures proteins and inactivates viral contaminants. Allows room temperature storage of DNA and safe sample transport and handling prior to purification
• High quality DNA. Enables full length PCR amplification
• Highly efficient method. Enables purification of sufficient DNA for up to 80 amplification reactions from a 50 µL blood sample
• No precipitation steps. DNA is ready for immediate analysis

This simple protocol uses a single micro centrifuge tube and small sample volume (5-50 µL) to produce high quality DNA for amplification by PCR. DNA for up to 80 amplification reactions can be obtained from a 50 µL fresh or anticoagulant-treated blood sample.

GenSpin incorporates patented FTA technology, which lyses cell and nuclear membranes on contact. The DNA is reversibly entrapped within this type of FTA filter matrix and can be stored for weeks at room temperature prior to purification. Cellular debris and proteins are removed by washing with GenSpin Buffer and TE-1 Buffer using a centrifuge. The purified DNA is released from this specialized filter matrix by heat elution and is ready for immediate PCR amplification.