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Ready-to-use single phase phenol solution for isolation of total RNA
Cat. No. AMR-N580-30ML
Cat. No. AMR-N580-100ML
Cat. No. AMR-N580-200ML
Features:
- High Yields of total RNA.
- High purity, intact total RNA in < 1 hour.
- Excellent results from even the most difficult of cells and tissues.
Compatible with a variety of downstream application:
- Northern analysis
- Dot blots
- Cloning
- In vitro translation of PolyA+ selected RNA
- RT-PCR
RiboZol™ Extracted RNA. Total RNA was extracted from K562 cells (1x106 cells) with RiboZol™ RNA Extraction Reagent. RNA pellets were resuspended in RiboReserve™ RNA storage buffer. Samples were electrophoresed on a 1.2% Agarose I™ formaldehyde gel. Lane I: RiboReady™ 1 Kb RNA Ladder; Lanes 2 & 3: RiboZol™ extracted RNA samples.
High yields of total RNA
Yields of RNA extracted with RiboZol™ are comparable to those obtained with a competitor’s extraction reagent.
RiboZol™ extracted RNA yields from 2 different cell lines. Data provided by Eunmi Ho, Catholic University, Korea. RNA was extracted from HeLa cell cultures (purple bars) 1x106 cells and from CaSki cultures (magenta bars) 1x106 cells according to standard procedures. Total RNA yields were determined by OD260
Excellent purity Purity of RNA extracted with RiboZol™ are comparable to those obtained with a competitor’s extraction reagent
Purity of RiboZol™ extracted RNA from 2 different cell lines. Data provided by Eunmi Ho, Catholic University, Korea. RNA was extracted from HeLa cell cultures (purple bars) 1x106 cells and from CaSki cultures (magenta bars) 1x106 cells according to provided procedures. RNA purity was determined by the OD260/280 ratio.
Compatibility with downstream applications
Tests of RiboZol™ extracted mRNA confirm that it is a fully suitable template for cDNA synthesis. In side-by-side comparisons, RiboZol™ extracted mRNA equaled results obtained with mRNA extracted with a competitor’s reagent
cDNA synthesis/PCR from RiboZol™ extracted RNA. Data provided by Beth Sullivan, PhD. Duke University Medical Center, Durham, NC. cDNA synthesis: mRNA was extracted from GM08854-mouse/ Human Chr21 hybrid cells with RiboZol™ or with a competitor’s reagent. 2 μg samples were added to SuperScript®III First Strand Synthesis System (Invitrogen) as templates for reverse transcription. PCR reactions: 0.5 μl of each cDNA was added to PCR reactions containing primers to mouse beta actin. Samples were removed after 35 cycles and loaded onto 2% agarose gels. Lane 1: 100 bp ladder; Lane 2: water; Lane 3: RiboZol™ RNA - RT; Lane 4: RiboZol™ RNA + RT; Lane 5: Competitor RNA - RT; Lane 6: Competitor RNA + RT. |
