The cDNA Integrity Kit offers an accurate means to evaluate cDNA preparations to determine the presence of full-length and extended transcripts prior to their use in RT-PCR, microarrays, differential display, and Northern analysis, and library construction. Results can be obtained from any single or double stranded cDNA sample from human, mouse, or rat cells or tissue.

The kit contains eight primer sets which amplify specific regions of four different, but commonly expressed genes under universal PCR conditions. Generation of product with the 3' primer set indicates that the gene is expressed; generation of product from the 5' primer set indicates full-length, intact cDNA.

Features and Benefits

• Conveniently utilizes universal PCR conditions to amplify target cDNA.
• Assures cDNA is representative of mRNA population.
• Saves time by verifying cDNA is full-length prior to downstream applications.
• Primers selection ensures greater sensitivity in the confirmation of cDNA quality, even low-expressed transcripts.

Kit Contents

• 5X PCR Buffer
• GAPDH 3’ Primer Set
• GAPDH 5’ Primer Set
• Clathrin 3’ Primer Set
• Clathrin 5’ Primer Set
• β-actin 3’ Primer Set
• β-actin 5’ Primer Set
• ARF F1 3’ Primer Set
• ARF F1 5’ Primer Set
• Control cDNA
• DEPC-treated Water

Recommended Storage Conditions
Stable for 1 year when stored at -20°C.