Cat. No. AMR-J556-1KIT
The LiGator Kit significantly reduces reaction times because it incorporates the theory of “molecular crowding.” This not only aids in the speed of the reaction, but also allows for more efficient cloning when using low concentrations of DNA. Additionally, by altering the
distribution of ligation products, intermolecular ligation (vector-to-vector, for example) is minimized. The Kit contains enough reagents to perform 30 ligation reactions.
Features:
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Complete Ligation Reactions in Just 15 Minutes
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Efficient Cloning at Low Concentrations of DNA
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Increased Productivity and Efficiency
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Kit Components:
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T4 DNA Ligase (4U/µl)
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10X Reaction Buffer
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Nuclease-Free Water
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Agarose 3:1 HRB
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Activity:
350ng of l Hind III digested DNA incubated with 1µl T4 DNA Ligase in 1X LiGator Reaction Buffer for 15 minutes at room temperature results in the visualization of band(s) of at least 21kb.
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