Proteomics blotter for rapid semi-dry blotting of proteins up to 150 KDa
Cat. No. BM-015-600
Features:
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Carbon based electrodes
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XXL sized blotter with 23.5 x 38.5 cm electrode size special designed for the transfer of big (2D) gels
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Passive cooling
Electro-blotting is an important method to transfer mainly proteins from polyacrylamide gels on to nitrocellulose or other carrier membranes. Semi-dry blotting is done between two
horizontal plate electrodes and offers fast as well as homogeneous transfer. In contrast to tank blotting only little transfer buffer is required and transfer times are shorter.
Additionally, with semi-dry blotting discontinuous buffer systems can be used to gently blot smaller proteins or to transfer protein mixtures of very different sizes more evenly.
The synthetic carbon electrodes of the new XXL-sized Fastblot B64 are highly alcali and oxidation resistant. The electrodes can be used for higher currents (up to 2.5
mA/cm2 gel for discontinuous buffer systems resp. up to 3.5 mA/cm2 gel for continuous buffer systems) so that blotting times are reduced to 10 to 30 minutes.
Large proteins (> 100 kDa) require longer transfer times. The heat that is generated by the extended transfer will be removed by passive cooling due to the special design of the
blotting apparatus. By applying higher current, proteins with higher molecular weights can be blotted faster and more quantitatively. Even smaller proteins can be transferred faster from
thick gels and gels with small pores when blotting with high current.
Nucleic acids can also be electro-blotted using the Fastblot systems. However, vacuum blotting is the method of choice for nucleic acids
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