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Minifold® I System
Fastblot B31 / B32 / B33 / B44 Superior 96- and 48-well manifolds for proteins & nucleic acids Dot-, Spot- and Slot-Blot Array Systems

The Minifold I system consists of four basic components: sample well plate, filter support plate, vacuum plenum, and metal clamping plate. The sample well plate is available in three configurations for producing spots, dots, or slots.

Features:

The Minifold I system is compatible with multichannel pipettes. All three plates are interchangeable and can be purchased as an accessory plate in conjunction with a complete system. The tension on the clamping is adjustable, permitting use of a variety of blotting and filtration media.

Minifold I Dot-Blot System - 96-Well

Unique O-ring design - ensures discrete dot formation without leakage of samples by cross lateral flow. Generates even, uniform dots which eliminates uneven test areas resulting from manual sample application.

Minifold I Spot-Blot System - 96-Well

Small volume required - sample volumes as low as 25 µL can be applied using less of your precious sample. Very high signal intensity - 2 mm 2 sample application area results in increased signal intensity. Standard microtitration format- 96 samples on a single membrane; same as the standard Minifold Dot-Blot system.

Minifold I Slot-Blot System - 48-Well

Preferred format for densitometric scanning - slots can be easily quantitated. Concentrated signal - 6 mm 2 sample application area results in high signal intensity. Easy to survey format - 48 samples on your membrane are easier to view than 96 samples.

Specifications:

 

Material

Filter Area

Max Capacity

Pressure

96-Well Dot-Blot Plate

Acrylic

12.5 mm 2/dot

500 µL/well, 96 wells

0.9 bar, vacuum

96-Well Spot-Blot Plate

Acrylic

2 mm 2/spot (1x2 mm)

200 µL/well, 96 wells

0.9 bar, vacuum

48-Well Slot-Blot Plate

Acrylic

6.24 mm 2/slot (7.8x0.8mm)

1000 µL/well, 48 wells

0.9 bar, vacuum


Description

Quantity/Pack

Cat. No.

Complete Systems

Minifold I Spot-Blot* System, complete 96-Well (Acylic)

1

W-10447850

Minifold I Dot-Blot* System, complete 96-Well (Acrylic)

1

W-10447900

Minifold I Slot-Blot* System, complete 48-Well (Acrylic)

1

W-10447941

Replacement Parts

Minifold I Spot-Blot plate

1

W-10447852

Minifold I Slot-Blot plate

1

W-10447906

Minifold I Dot-Blot plate

1

W-10447905

Minifold I Clamping plate

1

W-10447960

Vacuum Plenum

1

W-10447968

Accessory Products

Incubation plate

1

W-10447909

Cutting template

1

W-10447901

Membranes - 10.2 x 13.3 cm to fit Minifold I Systems

Protran®, BA79 0.1 µm

10

W-10402088

Protran, BA83 0.2 µm

10

W-10402488

Protran, BA85 0.45 µm

10

W-10402588

Optitran®, BA-S83 0.2 µm

10

W-10439388

Optitran, BA-S85 0.45 µm

10

W-10439188

Nytran® N, 0.45 µm

10

W-10416188

Nytran SuPerCharge, 0.45 µm

10

W-10416288

*Complete systems include: manifold apparatus, 5 sheets of Protran NC , 5 sheets of precut 3MM paper.

 

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