Superior 96- and 48-well manifolds for proteins & nucleic acids Dot-, Spot- and Slot-Blot Array
Systems
The Minifold I system consists of four basic components: sample well plate, filter support plate, vacuum plenum, and metal clamping plate. The sample well plate is available in three configurations for producing spots, dots, or slots.
Features:
The Minifold I system is compatible with multichannel pipettes. All three plates are interchangeable and can be purchased as an accessory plate in conjunction with a complete system. The tension on the clamping is adjustable, permitting use of a variety of blotting and filtration media.
Minifold I Dot-Blot System - 96-Well
Unique O-ring design - ensures discrete dot formation without leakage of samples by cross lateral flow. Generates even, uniform dots which eliminates uneven test areas resulting from manual sample application.
Minifold I Spot-Blot System - 96-Well
Small volume required - sample volumes as low as 25 µL can be applied using less of your precious sample. Very high signal intensity - 2 mm 2 sample application area results in increased signal intensity. Standard microtitration format- 96 samples on a single membrane; same as the
standard Minifold Dot-Blot system.
Minifold I Slot-Blot System - 48-Well
Preferred format for densitometric scanning - slots can be easily quantitated. Concentrated signal - 6 mm 2 sample application area results in high signal intensity. Easy to survey format - 48 samples on your membrane are easier to view than 96 samples.
Specifications:
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Material
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Filter Area
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Max Capacity
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Pressure
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96-Well Dot-Blot Plate
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Acrylic
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12.5 mm 2/dot
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500 µL/well, 96 wells
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0.9 bar, vacuum
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96-Well Spot-Blot Plate
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Acrylic
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2 mm 2/spot (1x2 mm)
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200 µL/well, 96 wells
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0.9 bar, vacuum
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48-Well Slot-Blot Plate
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Acrylic
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6.24 mm 2/slot (7.8x0.8mm)
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1000 µL/well, 48 wells
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0.9 bar, vacuum
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Description |
Quantity/Pack |
Cat. No. |
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Complete Systems |
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Minifold I Spot-Blot* System, complete 96-Well (Acylic) |
1 |
W-10447850 |
|
Minifold I Dot-Blot* System, complete 96-Well (Acrylic) |
1 |
W-10447900 |
|
Minifold I Slot-Blot* System, complete 48-Well (Acrylic) |
1 |
W-10447941 |
|
Replacement Parts |
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Minifold I Spot-Blot plate |
1 |
W-10447852 |
|
Minifold I Slot-Blot plate |
1 |
W-10447906 |
|
Minifold I Dot-Blot plate |
1 |
W-10447905 |
|
Minifold I Clamping plate |
1 |
W-10447960 |
|
Vacuum Plenum |
1 |
W-10447968 |
|
Accessory Products |
|
Incubation plate |
1 |
W-10447909 |
|
Cutting template |
1 |
W-10447901 |
|
Membranes - 10.2 x 13.3 cm to fit Minifold I Systems |
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Protran®, BA79 0.1 µm |
10 |
W-10402088 |
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Protran, BA83 0.2 µm |
10 |
W-10402488 |
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Protran, BA85 0.45 µm |
10 |
W-10402588 |
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Optitran®, BA-S83 0.2 µm |
10 |
W-10439388 |
|
Optitran, BA-S85 0.45 µm |
10 |
W-10439188 |
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Nytran® N, 0.45 µm |
10 |
W-10416188 |
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Nytran SuPerCharge, 0.45 µm |
10 |
W-10416288 |
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*Complete systems include: manifold apparatus, 5 sheets of Protran NC , 5 sheets of precut 3MM paper. |
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