100% Pure Nitrocellulose Membranes
Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible.

Features:
Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric and fluorescent.

Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wet in water, and then in the transfer buffer. No other pre-treatment steps are necessary.

High binding, low background
In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.
High retention of small proteins

The 0.2 µm pore size of the Protran (BA83) nitrocellulose membrane ensures high retention of small samples below 20 kD by reducing "blow-through". The 0.45 µm pore size membrane (BA85) is ideal for larger molecular weight samples. BA79, with 0.1 µm pore size, is the membrane of choice for smaller molecules below 7 kD.

A particular benefit of the proprietary Protran nitrocellulose formula is the proven excellent shelf life of proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran.