Designed for the separation and blotting of analytical and semi-preparative quantites of proteins in vertical 8.7 x 7.2 cm polyacrylamide (PAGE) gels
Features:
-
Runs 1 or 2 gels simultaneously
-
Patented drop-in wedge automatically positions gels without the use of gaskets or clamps
-
Electroblotting performed in the same buffer tank
The Mini-V system is an integrated system for rapid, high-quality separation and blotting of proteins (and nucleic acids) in a vertical mini-gel format. Its two primary components, the
Mini-V 8.10 Vertical Gel electrophoresis Apparatus and the Mini-V 8.10 Blot Module, are supplemented by a complete selection of accessories and replacement parts, including an external
gel casting system.
The Mini-V8.10 is designed for separation and blotting in a vertical 8.7 x 7.2 (W x L) cm mini-gel format. The patented drop-in wedge automatically positions gels without the use of
gaskets or clamps. Additional gels of different length and thickness can be electrophoresed. Most commercial available pre-cast 8 x 10 cm format PAGE gels can be used with the system. Up
to 20 samples can be separated by running 2 gels simultaneously. 3 sets of spacers with different thickness (0.5; 0.75 and 1.5 mm) allow optimal adaptation of gel thickness for various
applications.
The Mini-V8.10 Blot Module operates in the electrophoresis tank of the Mini-V8.10 for the transfer of proteins (and nucleic acids) to membranes. 1 or 2 gels can be blotted
simultaneously. The 1000 ml Puffer volume takes care for an efficient cooling even at higher current
It is recommended to use the Mini-V8.10 Gel Casting Clamp for simple, leak-free casting of gels. This allows casting of single gels in a fraction of the time of the "conventional" tape
or grease and binder clip methods.
The Mini-V8.10 Gel Casting System is designed for easy set-up and cleaning and allows to cast 2 to 9 gels simultaneously for increased reproducibility.
Specifications:
| Apparatus dimensions (W x D x H) |
16.7 x 14.0 x 15.0 cm |
| Gel size (W x L) |
8.7 x 7.2 cm |
| Glass plate size (W x L) |
10.25 x 7.25 / 8.25 cm |
| Number of gels |
1 or 2 |
| Capacity |
max. 10 samples per gel (20 samples total) |
| Typical sample volumes |
12 – 475 µl per well (depending on gel thickness) |
| Combs |
Thickness
(mm) |
Number of Wells |
Well Width (mm) |
Volume per Well
(µl) a |
| |
0.5
|
6
10
|
10.7
5.6
|
25
12
|
| |
0.75 |
6
10 |
10.7
5.6 |
40
20 |
| |
1.5
|
1 prep. + 2 marker lanes
6 10
|
63.4
10.7
5.6
|
475 c
80
40
|
| Buffer volume |
600 – 650 ml (total buffer volume) for electrophoresis 1,000 ml for blotting |
| |
|
a Volume calculated for an insertion depth of 5 mm
c Volume of the central preparative slot
|
Description
|
Cat. No.
|
|
Mini-V 8 · 10 Gel Electrophoresis System , complete system with Mini-V8 · 10 Gel Electrophoresis Apparatus and Mini-V8
· 10 Blot Module
(Note! The Mini-V8 · 10 Gel Casting Clamp is sold separately)
|
BM-21078118
|
|
|
|
|
Mini-V 8 · 10 Gel Electrophoresis Apparatus , complete with buffer tank, safety lid, gel support tray, gel wedge block, 2
pairs of glass plates (2 short glass plates 7.25 x 10.25 cm and 2 long glass plates 8.25 x 10.25 cm), 2 sets of 0.75 mm thick side spacers, 2 x 10 well combs (0.75 mm thick) and
manual
(Note! The Mini-V8 · 10 Gel Casting Clamp is sold separately.)
|
BM-21078092
|
|
|
|
|
Mini-V 8 · 10 Blot Module , complete with blot restrainer, blot support frame,
clamping knob, three transfer pressure pads and manual.
|
BM-21078019
|
|
|
|
|
Mini-V 8 · 10 Gel Casting System , complete with a casting chamber with
threaded posts, an end plate with silicone gasket and fill port, displacement
blocks (1 x 1 mm, 2 mm and 5 mm and 5 x 9 mm), 20 gel separation sheets, 10 pairs of glass plates, wing nuts and cap nuts, stopcock, tubing connector,
stopper and manual
(Note! Combs and spacers are sold separately!)
|
BM-21078027
|
|
