BCIP/NBT Phosphatase Substrate contains 5-bromo-4-chloro-3-indolyl-phosphate/nitroblue tetrazolium and is the most effective chromogenic substrate for immunoblot detection of phosphatase conjugates. Color formation occurs when alkaline phophatase catalyzes the dephophorylation of BCIP. This initiates a color cascade that produces crisp bands of intense purple precipitate with minimal background staining. BCIP/NBT is available as a 1-component and a 3-component system.

Product Features and Benefits

• Substrate stable for one year.
• Purple reaction product resists fading when exposed to light.
• Low background.

Kit Components:
3 Component BCIP/NBT Substrate

1. BCIP Concentrate Solution
2. NBT Concentrate Solution
3. Tris Buffer Solution

Recommended Storage Conditions:
Reagents are stable for 1 year when store at 2-8°C