TMB Membrane Peroxidase Substrates are the most sensitive chromogenic peroxidase substrate for use in membrane assays. TMB (3,3’,5,5’-tetramethylbenzidine) is non-carcinogenic and produces a stable, dark blue precipitate at the reaction site in the presence of peroxidase-labeled conjugates. It is optimized for use in membrane assays and dot blots. This ultrasensitive system significantly increases the detection limits of assays on a variety of membranes. TMB offters low nonspecific binding to the membrane and a long active shelf life compared to other membrane substrates. KPL offers TMB for membranes as a 1-component or 3-component system.

Product Features and Benefits

• Most sensitive chromogenic substrate: protein detection as low as 50 pg.
• Stable: liquid substrate stable for 1 year
• Minimal fading
• Safe: non-hazardous and non-carcinogenic

Kit Components: 
3-Component TMB Peroxidase Substrate:

1. TMB Peroxidase Substrate
2. Peroxidase Solution B
3. TMB Membrane Enhancer

Recommended Storage Conditions:
Reagents are stable for 1 year when store at 2-8°C