HistoMark® RED and HistoMark® BLUE systems contain reagents for the detection of alkaline phosphatase-labeled conjugates. HistoMark® RED reagents form a brilliant scarlet reaction product that is stable in organic solvents. Sections reacted with HistoMark® RED can be dehydrated through graded alcohols and xylene, then mounted in xylene-based mounting media for permanent documentation. The hematoxylin counterstain, Contrast BLUE, provides sharp contrast and excellent nuclear detail. HistoMark® BLUE reagents produce a royal blue reaction product that is alcohol soluble, and aqueous mounting media must be used. Contrast RED, a nuclear counterstain formulated from nuclear Fast Red, provides excellent nuclear detail.

Product Features and Benefits

• Stable liquid systems eliminate waste.
• Offer a variety of contrasting colors for double or triple staining protocols.
• Counterstain provides sharp contrast and improves nuclear detail.
• Convenient, easy-to-follow protocols.
• Contain levamisole that inhibits all endogenous alkaline phosphatases except for that from intestine.

Kit Contents
Sufficient reagents provided to process 1000 slides.

1. PhThaloRED or PhThaloBLUE Solution
2. Buffered Substrate Solution
3. Activator Solution
4. Contrast BLUE or Contrast RED

Recommended Storage Conditions
Stable for 1 year when stored at 2-8°C.