The ProteoSOL Tissue Extraction System enables the analysis of proteins in FFPE samples in a membrane-based immunoassay format. The kit includes reagents for the extraction of ten tissue samples, sample preparation tube, and a simple protocol with methods for tissue procurement using either needle dissection or laser capture microdissection. Tissue is extracted and ready-to-use in just 3 hours. Protein samples can be printed or spotted on nitrocellulose membranes and specific proteins can then be detected with standard colorimetric immunodetection protocols. Tissue extracts are sufficient for the preparation of multiple arrays and analysis of multiple proteins from the same tissue sample.

Features and Benefits

• Detect protein biomarkers in FFPE samples.
• Convenient array preparation and immunodetection.
• Fast: extracts are ready in just 3 hours.

Kit Contents

• ProteoSOL tissue extraction buffers and sample preparation tubes

Recommended Storage Conditions
Stable when stored at -20°C.

Number of tissue preparations	Reagents for extraction of 10 tissues
Tissue	FFPE samples from a variety of sources, including bladder, breast, colon, kidney, liver, lung, lymph node, ovary and prostate
Cell procurement	Protocol provided for needle dissection and for laser capture microdissection of tissue; optimized for approximately 60, 000 cells
Extraction and preparation time	Tissue is extracted and ready to use in just 3 hours
Array preparation	Sufficient for preparation of multiple arrays and analyzing multiple proteins from the same tissue sample
Immunodetection	Compatible with KPL immunoblot reagents, antibody conjugates and colorimetric substrates

Description	Size	Cat. No.
ProteoSOL Tissue Extraction System	10 reactions	KP-554-00-02

This product is manufactured and sold under a liscense from Expression Pathology Inc., Gaithersburg, MD, and incorporates Expression Pathology’s proprietary Liquid TissueTM reagents for extraction of biomolecules from formalin-fixed paraffin-embedded tissue.

Trademarks: ProteoSOL is a trademark of KPL, Inc. Liquid Tissue is a trademark of Expression Pathology Inc.