Protein Detector™ AP ELISA Kits provide pretested components to reduce the time and effort needed to develop a sensitive and reproducible assay. All components needed to establish an ELISA except primary antibody and plates are provided. A comprehensive manual to facilitate the development of enzyme immunoassay procedures is also included. KPL's protein Detector™ AP ELISA Kits are also provided with BluePhos® Phosphatase Substrate that produces an intense blue color measurable between 595 and 650 nm. BluePhos® offers significant advantages over pNPP including greater sensitivity, wider dynamic range, and lower background.

Protein Detector™ AP ELISA Kits permit rapid, sensitive immunodetection of protein in microwell plates. They also provide a convenient means of analyzing hybridoma cultures for the presence of antibodies of desired specificity. With the appropriate choice of labeled antibody, this kit can be used to identify antigen-specific mouse, human and rabbit antibodies.

Product Features and Benefits

• Versatile: direct and indirect sandwich assays; hybridoma screening; stopped, nonstopped and kinetic assays.
• Pretested wash, coating, and blocking solutions ensure low background.
• Kit provides highly sensitive reagents optimized for detection of pictogram quantities of antigen.
• Reagents packaged to reduce waste and minimize preparation time.

Kit Components:
1. Coating Solution Concentrate
2. BSA Diluent/Blocking Solution Concentrate
3. Wash Solution Concentrate
4. 50% Glycerol Solution
5. AP labeled Goat Anti-Human IgG (H+L)
6. BluePhos® Phosphatase Substrate System
7. BluePhos® Stop Solution Concentrate