SureLINK Chromophoric Biotin Labeling Kits provide a quick and reliable method for labeling antibodies and other proteins with biotin and enable easy measurement of biotin incorporation in the conjugate.  Proteins and peptides can be readily labeled with biotin through the modification of primary amines.

The advantages of SureLINK Biotin for quantitating biotin incorporation contrast sharply with the limitations of the standard HABA method.  Figure 1 shows a comparison of SureLINK with two commercially available HABA kits from two different suppliers. It clearly demonstrates the reproducibility of biotin quantitation with SureLINK as opposed to the variability of HABA, especially at low protein concentrations. 

Figure 1: A single biotin conjugate of mouse monoclonal antibody was prepared using the SureLINK Chromophoric Biotin Labeling Kit.  The conjugate was serially diluted and biotin content tested using commercially available HABA kits and by direct measurement of the chromophoric biotin at A354.

These kits contain an activated form of biotin that carries a unique chromophore, reconstitution solvent, modification buffer and spin filters for purifying biotin conjugates.  Each 0.5 mg vial of biotin is sufficient for labeling approximately 1.5 - 10 mg protein.  Kit components are stable for one year when stored
at 2-8°C.