Western blotting from transfer to development in under an hour - No expensive equipment required!

• Streamline each step of your Western Blotting procedure with AMRESCO's Rapid Western Blotting Kit.
• Versatile kits include all reagents (except for user supplied primary antibodies).
• No expensive, additional transfer equipment is needed.
• All reagents are protein-free formulations to minimize cross-reactivity and enhance antigen availability.
• Kits are supplied for either standard wet or semi-dry transfer apparatus.
• Offered with either anti-mouse or anti-rabbit secondary antibodies that are optimized to generate blots with low backgrounds and reduced blotches.
  

Rapid Transfer:

• Transfer from gel to membrane in 10-20 minutes
• Compatible with standard wet or semi-dry transfer apparatus
• Compatible with PVDF and nitrocellulose membranes

Blocking:

• Membrane blocking in 5 minutes
  

Antibody Incubation:

• Combined primary and secondary antibody binding step reduces total incubation times to 30-45 minutes
• Available with either anti-mouse or anti-rabbit secondary antibodies
• Secondary antibodies bind user-supplied monoclonal or polyclonal primary antibodies

Washes:

• Reduced to a total of 15 minutes
  

HSP90 and B-Tubulin detection with AMRESCO's Rapid Western Blot Kit compared to a competitor's one-hour Western Blot kit. 10 µg per lane of cytoplasmic proteins isolated from K562 cells with AMRESCO's Cytoplasmic & Nuclear Protein Enrichment Kit (M330) were resolved on a 12.5% Fluorescent Sprint Next Gel(M318). Bands were visualized on a Syngene G-box transilluminator with a ethidium bromide filter. Proteins were transferred onto PVDF membranes with a semi-dry transfer unit for 10 minutes in Rapid Transfer Buffer (N789). The blot was blocked, cut into strips and stained with HSP90 and β-tubulin antibodies with either AMRESCO's Rapid Western Blot Kit or a competitor's one-hour Western Blot Kit. Distinct bands were observed using the Rapid Western Blot Kit, while no signal could be detected using the competitor's kit.

Each Rapid Western Blotting Kit includes:

• Rapid Transfer Buffer, 10X
• RapidBlock™, 10X
• Rapid Blot Antibody Diluent, 10X
• Rapid Wash Buffer, 20X
• Rapid Blot Secondary HRP Antibody, available as anti-mouse or anti-rabbit
• Sufficient material for 15 blots

Available Separately
Kit components available separately include:

• Rapid Transfer Buffer (AMR-N789)
• RapidBlock™ (AMR-M325)