SureLINK AP Conjugation Kits let you take the work and guesswork out of protein labeling!Based on a novel conjugation chemistry that overcomes many limitations of other conventional methods of bioconjugation, such as maleimide/thio and glutaraldehyde. SureLINK AP Conjugation Kits enable protein labeling with alkaline phosphatase in only 3 hours, saving you several hours over standard maleimide/thiol methods. Hands-on time is minimal, just 20 minutes. The kits provide Modified AP that is ready for conjugation, ready-to-use buffers, and optimized storage buffer.

SureLINK Modified AP is a modified form of alkaline phosphatase (AP) ideal for conjugating monoclonal antibodies or other proteins. AP has been modified with the linker succinimidyl r-hydrazinonicotinate (SANH). Based on unique conjugation chemistry, the Modified AP is part of a two-step coupling reaction. The conjugation reaction takes approximately 2 hours to complete.

Features:

• Simple, 2-step protocol is complete with only 20 minutes hands-on time
• Minimal optimization – Avoids problems such as polymerization and self-conjugation
• Highly-specific binding – Novel chemistry virtually eliminates nonspecific reactions
• Versatile and convenient – Kits can be used to conjugate a range of protein sample sizes
• Ideal for immunodetection – Antibodies or proteins labeled with SureLINK AP can be used in ELISA, Western blotting, and immunohistology or other protein applications

Kit components:

Each kit contains SureLINKModifiedAP in individual, ready-to-use vials, AP Modification Buffer, AP Conjugation Buffer, SFB Linker in individual vials, and AP Storage Buffer. All kit components are stored at 2-8°C and are stable for one year when stored as recommended.