 GracePure™ SPE silica-based sorbents are characterized with over 20 tests to ensure unvarying selectivity and the highest lot-to-lot reproducibility. Packed SPE columns have less than 2% bed weight variation. This combination means GracePure™ removes variables associated with product, and lets you concentrate on the method development process. GracePure™ SPE products have an extensive offering of sorbents suitable for a variety of applications. These products deliver selectivity and high recovery, ensuring a cleaner, more concentrated sample at the end of the SPE process.
Application Guide: Use this guide to help you choose the appropriate sorbent, bed size, and solvent volumes to fit your application.
Reversed Phase:
Sorbent |
Support |
Carbon (%) |
Endcapping |
Surface Area (m2/g) |
Particle
Size (μm) |
Pore Size (Å) |
Feature |
Benefit |
Application |
C18-Max |
Silica |
17 |
Yes |
518 |
50 |
60 |
Polymerically bonded
17% carbon load |
Highest binding capacity,
best for complex samples or structurally diverse analytes. |
Drugs and their metabolites
in serum and plasma,
pesticides |
C18-Aq |
Silica |
12.5 |
Yes |
518 |
50 |
60 |
Hydrophilic
endcapping |
Water-wettable C18 ideal for aqueous samples Phase remains active even when completely dry. |
Desalting proteins,
pharmaceuticals, hormones, pesticides, organics in water |
C18-300 |
Silica |
8.5 |
No |
100 |
13 |
300 |
Large 300Å pore size |
Retains proteins and
peptides. |
Proteins, peptides, desalting, DNA, large biomolecules, removal of HF and cleavage
products |
C18-Low |
Silica |
6.5 |
Yes |
518 |
50 |
60 |
Least hydrophobic C18 phase |
C18 phase that easily
releases very hydrophobic
compounds. |
Surfactants, oils, antibiotics |
C18-Fast |
Silica |
7.0 |
Yes |
518 |
100 |
60 |
Large 100μm particle |
Process large volume
(>500mL) or viscous samples with fast flow rates. |
Aniline, pesticides,
haloethers, phalate esters,
EPA 3620, 3610 |
TMS |
Silica |
5.6 |
No |
518 |
50 |
60 |
Low carbon load
trimethyl silane phase |
Least hydrophobic reversed phase elutes non-polar compounds easily. Short carbon chain has little steric hindrance to uniformly cover silica surface. |
Oils, dyes, surfactants |
Normal Phase:
Sorbent |
Support |
Carbon (%) |
Endcapping |
Surface Area (m2/g) |
Particle
Size (μm) |
Pore Size (Å) |
Feature |
Benefit |
Application |
Silica |
Silica |
N/A |
N/A |
518 |
50 |
60 |
Most polar phase |
Able to differentiate between structurally similar
compounds. |
Aflatoxins, pesticides, steroids, structural isomers |
Amino |
Silica |
4.3 |
No |
518 |
50 |
60 |
Duel retention |
Retains polar compounds, or can act as a weak anion exchanger. Easily releases strong acids when SAX binds
too strongly. |
Carbohydrates, dyes, lipids, mycotoxins, strong acids |
Diol |
Silica |
N/A |
No |
518 |
50 |
60 |
Reproducible polar bonded phase |
Very polar phase that has the same benefits as silica, but wets easily and offers more
reproducibility. |
Alkaloids, lipids, oils, structural isomers |
Ion Exchange:
Sorbent |
Support |
Exchange
Capacity
(meq/g) |
Counter
Ion |
Particle
Size
(μm) |
Feature |
Benefit |
Application |
Anion-X |
8% crosslinked
styrenedivinylbenzene |
1.5 |
Acetate form |
50 |
Tetramethyl
ammonium functional
group on polymer
base material |
pH range from 1- 14, with excellent exchange capacity. |
Anionic compounds: organic acids, fatty acids |
Cation-X |
8% crosslinked
styrenedivinylbenzene |
2.4 |
Hydrogen form |
50 |
Benzene sulfonic acid
functional group on
polymer base material |
pH range from 1- 14, with excellent exchange capacity. |
Cationic compounds: amines, amino acids |
Sorbent |
Bed Size/Tube Volume |
Qty. |
Cat. No. |
C18-Max |
100mg/1mL |
100pk |
A-5138765 |
C18-Max |
500mg/3mL |
50pk |
A-5138766 |
C18-Max |
500mg/6mL |
30pk |
A-5138767 |
C18-Max |
1000mg/6mL |
30pk |
A-5138768 |
C18-Aq |
100mg/1mL |
100pk |
A-5138774 |
C18-Aq |
500mg/3mL |
50pk |
A-5138775 |
C18-Aq |
1000mg/6mL |
30pk |
A-5138776 |
C18-300 |
100mg/1mL |
100pk |
A-5138756 |
C18-300 |
500mg/3mL |
50pk |
A-5138757 |
C18-Low |
100mg/1mL |
100pk |
A-5135760 |
C18-Low |
200mg/3mL |
50pk |
A-5138761 |
C18-Low |
500mg/3mL |
50pk |
A-5138762 |
C18-Low |
500mg/6mL |
30pk |
A-5138763 |
C18-Low |
1000mg/6mL |
30pk |
A-5138764 |
C18-Fast |
500mg/3mL |
50pk |
A-5138758 |
C18-Fast |
1000mg/6mL |
30pk |
A-5138759 |
TMS |
100mg/1mL |
100pk |
A-5138785 |
TMS |
500mg/3mL |
50pk |
A-5138786 |
Silica |
100mg/1mL |
100pk |
A-5138777 |
Silica |
200mg/3mL |
50pk |
A-5138778 |
Silica |
500mg/3mL |
50pk |
A-5138779 |
Silica |
500mg/6mL |
30pk |
A-5138780 |
Silica |
1000mg/6mL |
30pk |
A-5138781 |
Silica |
2000mg/12mL |
30pk |
A-5138782 |
Silica |
5000mg/20mL |
20pk |
A-5138783 |
Silica |
10000mg/60mL |
16pk |
A-5138784 |
Amino |
500mg/3mL |
50pk |
A-5138752 |
Amino |
1000mg/6mL |
30pk |
A-5138753 |
Diol |
100mg/1mL |
100pk |
A-5138771 |
Diol |
200mg/3mL |
50pk |
A-5138772 |
Diol |
500mg/3mL |
50pk |
A-5138773 |
Anion-X |
100mg/1mL |
100pk |
A-5138754 |
Anion-X |
500mg/3mL |
50pk |
A-5138755 |
Cation-X |
100mg/1mL |
100pk |
A-5138769 |
Cation-X |
500mg/3mL |
50pk |
A-5138770 |
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